Despite the availability of a large number of antibody and DNA based methods for detection and quantification of allergens in food there remain significant difficulties in selecting the optimum technique to employ.
Published methods from research groups mostly contain sufficient detail concerning target antigen, calibration procedures and method performance to allow replication by others.
 CRSP-1 |
|
B5452-.1 |
ApexBio |
100 ug |
EUR 415 |
 ADWX 1 |
|
B5722-.1 |
ApexBio |
100 ug |
EUR 583 |
|
Description: ADWX 1 is a potent and selective inhibitor of KV1.3 channel with IC50 values of 1.89 pM and 0.65 nM for Kv1.3 and Kv1.1, respectively [1]. |
 Purotoxin 1 |
|
B5771-.1 |
ApexBio |
100 µg |
EUR 380 |
 Psalmotoxin 1 |
|
B5796-.1 |
ApexBio |
100 ug |
EUR 447 |
|
Description: IC50: 0.9 nMPsalmotoxin 1 is a potent and selective acid-sensing ion channel 1a (ASIC1a) blocker.Several ASIC subunits described: ASIC1a, ASIC1b, ASIC2a, ASIC2b, and ASIC3 with different kinetics, tissue distribution, and external pH sensitivities. |
 Rbin-1 |
|
B7823-1 |
ApexBio |
1 mg |
EUR 154 |
|
Description: Rbin-1 is an eukaryotic ribosome assembly inhibitor.All cellular proteins are synthesized by ribosomes, whose biogenesis is a complex multi-step process quickly completed in eukaryotes. |
 UK 1 |
|
C5006-1 |
ApexBio |
1 mg |
EUR 363 |
|
Description: UK 1, an unusual bis-benzoxazole metabolite isolated from Streptomyces sp. 517-02, is an inhibitor of topoisomerase II (Topo II) and hepatitis C viral replication. |
 KP372-1 |
|
C5053-1 |
ApexBio |
1 mg |
EUR 132 |
|
Description: IC50: 30-60 nM for thyroid cancer cells KP372-1 is a specific Akt inhibitor. The phosphatidylinositol 3' kinase (PI3K)/phosphatase, which is a key regulator of cell proliferation and survival, is mutated or activated in various cancers. |
 Flutax 1 |
|
B6986-1 |
ApexBio |
1 mg |
EUR 399 |
) Anti-HO-1 Monoclonal Antibody (HO-1-2) |
|
M00253-1 |
BosterBio |
1mg |
EUR 397 |
|
Description: Mouse Monoclonal HO-1 Antibody (HO-1-2). Validated in Flow Cytometry, IHC, WB and tested in Human, Mouse, Rat. |
 IL-1-beta Interleukin-1 betaHuman Recombinant Protein |
|
PROTP01584-1 |
BosterBio |
Regular: 10ug |
EUR 317 |
|
Description: Interleukin-1 beta Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 153 amino acids and having a molecular mass of 17000 Dalton.;The IL-1b is purified by proprietary chromatographic techniques. |
 MMP-1 Matrix Metalloproteinase-1 Human Recombinant Protein |
|
PROTP03956-1 |
BosterBio |
Regular: 20ug |
EUR 317 |
|
Description: MMP 1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 393 amino acids (100-469a.a) and having a molecular mass of 45kDa. MMP 1 is fused to a 23 amino acid His-tag at N-terminus. |
 CDK7-IN-1 |
|
B4665-1 |
ApexBio |
1 mg |
EUR 358 |
 LPA2 antagonist 1 |
|
B4726-1 |
ApexBio |
1 mg |
EUR 319 |
 PACAP 1-27 |
|
B5075-1 |
ApexBio |
1 mg |
EUR 290 |
) Nociceptin (1-7) |
|
B5087-1 |
ApexBio |
1 mg |
EUR 399 |
) Hemokinin 1 (mouse) |
|
B5127-1 |
ApexBio |
1 mg |
EUR 321 |
) GIP (1-39) |
|
B5280-1 |
ApexBio |
1 mg |
EUR 609 |
 JMV 390-1 |
|
B5317-1 |
ApexBio |
1 mg |
EUR 242 |
) Hemokinin 1 (human) |
|
B5318-1 |
ApexBio |
1 mg |
EUR 340 |
 Echistatin, ?1 isoform |
|
B5391-.1 |
ApexBio |
100 ug |
EUR 583 |
 PACAP 1-38 |
|
B6625-1 |
ApexBio |
1 mg |
EUR 347 |
 SL 0101-1 |
|
B9008-1 |
ApexBio |
1 mg |
EUR 163 |
 Teicoplanin A3-1 |
|
C4943-1 |
ApexBio |
1 mg |
EUR 326 |
|
Description: Teicoplanin A3-1, also known as Antibiotic L 17054, is a glycopeptide antibiotic derived from Actinoplanes teichomyceticus and produces a potent broad spectrum antibiotic activity against gram-positive bacteria. |
 IDO-IN-1 |
|
C3211-1 |
ApexBio |
1 mg |
EUR 139 |
|
Description: IC50: 59 nMIDO-IN-1 is an indoleamine-2,3-dioxygenase (IDO) inhibitor. |
) RN-1 (hydrochloride) |
|
C4247-1 |
ApexBio |
1 mg |
EUR 112 |
|
Description: RN-1 (hydrochloride) is a brain-penetrant, potent and irreversible LSD1 inhibitor with IC50 values of 10-70 nM [1]. Lysine-specific demethylase 1 (LSD1) is a flavin-dependent monoamine oxidase that demethylate mono- and di-methylated lysines, specifically histone 3, lysines 4 and 9. |
) StemRegenin 1 (hydrochloride) |
|
C3551-1 |
ApexBio |
1 mg |
EUR 118 |
|
Description: IC50: 127 nM in CD34+ cellsStemRegenin 1 is an aryl hydrocarbon receptor signaling antagonist.The aryl hydrocarbon receptor (AhR ) is a protein that is encoded by the AHR gene. |
 HPGDS inhibitor 1 |
|
B1046-1 |
ApexBio |
1 mg |
EUR 181 |
|
Description: HPGDS inhibitor 1 is an oral potent and selective inhibitor of hematopoietic prostaglandin D synthase (HPGDS) with IC50 value of 0.7nM [1].Prostaglandin D2 (PGD2) is a mediator of allergy and inflammation. |
 Sphingosine-1-phosphate |
|
B6707-1 |
ApexBio |
1 mg |
EUR 181 |
|
Description: Sphingosine-1-phosphate is an endogenous second messenger that involved in cell proliferation and survival and is a ligand for S1PR1 [1] [2]. |
 TASIN-1 hydrochloride |
|
B2088-1 |
Biovision |
|
EUR 142 |
 RAD51D (1-328 a.a.) Human Recombinant Protein) (1-328) RAD51D (1-328 a.a.) Human Recombinant Protein |
|
PROTO75771-1 |
BosterBio |
Regular: 10ug |
EUR 317 |
|
Description: RAD51D (1-328) Human Recombinant produced in E. coli is. a single polypeptide chain containing 351 amino acids and having a molecular mass of 37.4kDa. RAD51D (1-328) is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
 XMIR-1 RNA oligo miRNA-1-3p with Xmotif |
|
XMIR-1 |
SBI |
10 rxn |
EUR 438 |
|
|
 Anti-BOB-1/OBF-1 Rabbit Monoclonal Antibody, Clone#RM378 |
|
M04431-1 |
BosterBio |
100uL |
EUR 385 |
|
Description: Anti-BOB-1/OBF-1 Rabbit Monoclonal Antibody, Clone#RM378 tested in WB, IHC, reactive to Human |
 TGF-b-1 Transforming Growth Factor-beta 1 Human protein |
|
PROTP01137-1 |
BosterBio |
Regular: 2.5ug |
EUR 1157 |
|
Description: Human Transforming Growth Factor-beta 1 purified from Human Platelets having a molecular mass of 25kDa.;The TGF-b 1 is purified by proprietary chromatographic techniques. |
) MCP-1 Monocyte Chemotactic Protein-1 Mouse Recombinant Protein (CCL2) |
|
PROTP10148-1 |
BosterBio |
Regular: 10ug |
EUR 317 |
|
Description: Monocyte Chemotactic Protein-1 Mouse Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 14 kDa. ;The MCP-1 is purified by proprietary chromatographic techniques. |
 Envelope-1, Dengue Virus Subtype 1 Recombinant Protein, Insect Cells |
|
PROTB1PNJ5-1 |
BosterBio |
Regular: 10ug |
EUR 317 |
|
Description: Recombinant Dengue Virus Subtype 1 produced in Insect Cells is a polypeptide chain containing amino acids 281-675 and having a molecular weight of approximately 50kDa. ;Dengue Envelope-1 is purified by proprietary chromatographic technique. |
 AssayMax ELISA Kit) Human Interleukin-1 beta (IL-1 beta) AssayMax ELISA Kit |
|
EI2200-1 |
AssayPro |
96 Well Plate |
EUR 477 |
 AssayMax ELISA Kit) Human Interleukin-1-alpha (IL-1-alpha) AssayMax ELISA Kit |
|
EI2301-1 |
AssayPro |
96 Well Plate |
EUR 477 |
 AssayMax ELISA Kit) Human KRAB-associated Protein 1 (KAP-1) AssayMax ELISA Kit |
|
EK2802-1 |
AssayPro |
96 Well Plate |
EUR 477 |
 AssayMax ELISA Kit) Mouse Interleukin-1 beta (IL-1 beta) AssayMax ELISA Kit |
|
EMI2200-1 |
AssayPro |
96 Well Plate |
EUR 477 |
 AssayMax ELISA Kit) Human Plasminogen Activator Inhibitor-1 (PAI-1) AssayMax ELISA Kit |
|
EP1100-1 |
AssayPro |
96 Well Plate |
EUR 417 |
) MCP-1 Monocyte Chemotactic Protein-1 Human Recombinant Protein (CCL2) |
|
PROTP13500-1 |
BosterBio |
Regular: 20ug |
EUR 317 |
|
Description: Monocyte Chemotactic Protein-1 Human Recombinant also known as Monocyte Chemotactic and Activating Factor (MCAF) produced in E.Coli is a non-glycosylated, Polypeptide chain containing 76 amino acids and having a molecular mass of 8.6kDa. ;The MCP-1 is purified by proprietary chromatographic techniques. |
 GAD1 iso1 Glutamate Decarboxylase 1 Isoform-1 Human Recombinant Protein |
|
PROTQ99259-1 |
BosterBio |
Regular: 20ug |
EUR 317 |
|
Description: GAD1 iso1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 617 amino acids (1-594 a.a) and having a molecular mass of 69.3kDa. GAD1 iso1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
 AXMIR-1 RNA oligo anti-miRNA-1-3p with Xmotif |
|
AXMIR-1 |
SBI |
10 reactions |
EUR 438 |
|
|
 Anti-PARP-1 Antibody |
|
A00122-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal PARP-1 Antibody. Validated in ELISA, IP, IF, WB and tested in Human, Mouse. |
 Anti-Presenilin 1 Antibody |
|
A00138-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for Presenilin 1 Antibody (PSEN1) detection. Tested with WB, IHC in Human, Mouse, Rat. |
 Anti-CNG-1 Antibody |
|
A05494-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for CNG-1 Antibody (CNGA1) detection. Tested with WB in Human, Mouse, Rat. |
 Anti-Periphilin 1 Antibody |
|
A06996-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for Periphilin 1 Antibody (PPHLN1) detection.tested for WB in Human, Mouse. |
 Anti-Lyl-1 Antibody |
|
A07491-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Lyl-1 Antibody. Validated in IHC and tested in Human, Mouse, Rat. |
 Anti-GLI-1 Antibody |
|
A07972-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for GLI-1 Antibody (ACSS1) detection. Tested with WB in Human, Mouse, Rat. |
 Anti-Cerebellin 1 Antibody |
|
A09176-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for Cerebellin 1 Antibody (CBLN1) detection. Tested with WB in Human, Mouse, Rat. |
 Anti-DOC-1 Antibody |
|
A09467-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for DOC-1 Antibody (CDK2AP1) detection. Tested with WB in Human, Mouse. |
 Anti-PAI-1 Antibody |
|
A00637-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for PAI-1 Antibody (SERPINE1) detection.tested for WB in Human, Mouse, Rat. |
 Anti-Flk-1 Antibody |
|
A00901-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for Flk-1 Antibody (KDR) detection.tested for WB in Human, Mouse. |
 Anti-EDG-1 Antibody |
|
A01502-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for EDG-1 Antibody (S1PR1) detection.tested for WB in Human, Mouse, Rat. |
 Anti-ROBO-1 Antibody |
|
A01530-1 |
BosterBio |
100ug |
EUR 455 |
|
Description: Rabbit Polyclonal ROBO-1 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat. |
 Bovine LIVER 1 EA* |
|
57123-1 |
Pel-Freez |
1 Each |
EUR 180.28 |
 Anti-NPDC-1 Antibody |
|
A12846-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for NPDC-1 Antibody (NPDC1) detection. Tested with WB in Human, Mouse, Rat. |
 Anti-Bag-1 Antibody |
|
A02423-1 |
BosterBio |
50 ul |
EUR 397 |
|
Description: Rabbit Polyclonal Bag-1 Antibody. Validated in IP, IHC and tested in Bovine, Canine, Human, Mouse, Rat. |
 Anti-TUB 1 Antibody |
|
A02917-1 |
BosterBio |
100ug/vial |
EUR 294 |
 Anti-Dok-1 Antibody |
|
A03039-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for Dok-1 Antibody (DOK1) detection.tested for WB in Human, Mouse, Rat. |
 Anti-TFIIIB90-1 Antibody |
|
A03761-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for TFIIIB90-1 Antibody (BRF1) detection.tested for WB in Human, Mouse. |
 Anti-Atrophin-1 Antibody |
|
A03828-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for Atrophin-1 Antibody (ATN1) detection. Tested with WB in Human, Mouse, Rat. |
 Supercoiled pHOT-1 DNA |
|
TG2030-1 |
TopoGen |
25 ug |
EUR 246 |
 Relaxed pHOT-1 DNA |
|
TG2035-1 |
TopoGen |
25 ug |
EUR 246 |
) Beta-Amyloid (1-11) |
|
A1002-1 |
ApexBio |
1 mg |
EUR 102 |
|
Description: Beta-amyloid (1-11) (Abeta or A?) (C56H76N16O22) is a peptide with the sequence H-{Asp}{Ala}{Glu}{Phe}{Arg}{His}{Asp}{Ser}{Gly}{Tyr}{Glu}-OH,which is processed from the Amyloid precursor protein. |
) Amyloid ?-Protein (1-15) |
|
A1003-1 |
ApexBio |
1 mg |
EUR 113 |
|
Description: Beta-amyloid protein (A beta), a 39-43 amino acid peptide composed of a portion of the transmembrane domain and the extracellular domain of the amyloid precursor protein (APP), is also the principal component of amyloid. |
, rat) ANP (1-11), rat |
|
A1009-1 |
ApexBio |
1 mg |
EUR 84 |
|
Description: Atrial Natriuretic Peptide (ANP) (C49H84N20O15S) has the sequence of H-Ser-Leu-Arg-Arg-Ser-Ser-Cys-Phe-Gly-Gly-Arg-OH, is a 28 amino acid peptide that is synthesized, stored, and released by atrial myocytes in response to atrial distension, endothelin, and sympathetic stimulation. |
 alpha-1 antitrypsin fragment |
|
A1051-1 |
ApexBio |
1 mg |
EUR 108 |
|
Description: Alpha-1 antitrypsin (A1AT) (H2N-Leu-Gln-His-Leu-Glu-Asn-Glu-Leu-Thr-His-Asp-Ile-Ile-Thr-Lys-OH), which is known as serum trypsin inhibitor, is a protease inhibitor belonging to the serpin superfamily. |
, human) BNP (1-32), human |
|
A1105-1 |
ApexBio |
1 mg |
EUR 177 |
|
Description: Basic natriuretic peptide (BNP), now known as B-type natriuretic peptide (also BNP) or GC-B, is a 32 amino acid polypeptide secreted by the ventricles of the heart in response to excessive stretching of heart muscle cells (cardiomyocytes). |
 B-Raf IN 1 |
|
B4727-1 |
ApexBio |
1 mg |
EUR 118 |
NH2) Nociceptin (1-13)NH2 |
|
B5102-1 |
ApexBio |
1 mg |
EUR 184 |
) JIP-1 (153-163) |
|
B5130-1 |
ApexBio |
1 mg |
EUR 399 |
) Nogo-66 (1-40) |
|
B5247-1 |
ApexBio |
1 mg |
EUR 609 |
) Orphanin FQ (1-11) |
|
B5497-1 |
ApexBio |
1 mg |
EUR 226 |
 R-1 Methanandamide Phosphate |
|
C3352-1 |
ApexBio |
1 mg |
EUR 126 |
|
Description: R-1 Methanandamide Phosphate,a water soluble prodrug analog of AEA, exhibited similar activity to that of AEA in the growth inhibition of C6 glioma cells[1]. Arachidonoyl ethanolamide (AEA) was the first endogenous cannabinoid (CB). |
However, routine allergen testing by the food industry relies upon commercialised test kits and frequently the suppliers provide disappointingly little specification detail on the grounds that this is proprietary information. In this review we have made a critical assessment of the published literature describing the performance of both commercial and non-commercial test kits for food allergens over the period 2008-2018.
Mass spectrometric methods, which have the potential to become reference methods for allergens, are not covered in this review. Available information on the specifications of commercial ELISA and LFD test kits are tabulated for milk, egg and peanut allergens, where possible linking to publications concerning collaborative studies and proficiency testing.
For a number of commercial PCR test kits, specifications provided by manufacturers for detection of a small selection of allergen are tabulated. In conclusion we support the views of others of the critical need for allergen reference materials as the way forward to improve the comparability of different testing strategies in foods.
