Multicenter Evaluation of the C6 Lyme ELISA Kit for the Diagnosis of Lyme Disease.

Multicenter Evaluation of the C6 Lyme ELISA Kit for the Diagnosis of Lyme Disease.

Lyme disease (LD), caused by infection with Borrelia burgdorferi, is the most common tick-borne infection in many regions of Eurasia. Antibody detection is the most frequently used laboratory test, favoring a two-step serodiagnostic algorithm; immunoenzymatic detection of antibodies to C6 has been shown to perform similarly to a standard two-step workflow.
The aim of this study was the performance evaluation of the C6 Lyme ELISA kit compared to a standard two-step algorithm in three laboratories located in the northeastern region of Italy which cater to areas with different LD epidemiology. A total of 804 samples were tested, of which 695 gave concordant results between C6 testing and routine workflow (564 negative, 131 positive).
Wherever available, clinical presentation and additional laboratory tests were analyzed to solve discrepancies. The C6 based method showed a good concordance with the standard two-step algorithm (Cohen’s κ = 0.619), however, the distribution of discrepancies seems to point towards a slightly lower specificity of C6 testing, which is supported by literature and could impact on patient management. The C6 ELISA, therefore, is not an ideal stand-alone test; however, if integrated into a two-step algorithm, it might play a part in achieving a sensitive, specific laboratory diagnosis of LD.

Development of a Direct Competitive ELISA Kit for Detecting Deoxynivalenol Contamination in Wheat.

This study was conducted to develop a self-assembled direct competitive enzyme-linked immunosorbent assay (dcELISA) kit for the detection of deoxynivalenol (DON) in food and feed grains. Based on the preparation of anti-DON monoclonal antibodies, we established a standard curve with dcELISA and optimized the detection conditions. The performance of the kit was evaluated by comparison with high-performance liquid chromatography (HPLC).
The minimum detection limit of DON with the kit was 0.62 ng/mL, the linear range was from 1.0 to 113.24 ng/mL and the half-maximal inhibition concentration (IC50) was 6.61 ng/mL in the working buffer; there was a limit of detection (LOD) of 62 ng/g, and the detection range was from 100 to 11324 ng/g in authentic agricultural samples. We examined four samples of wheat bran, wheat flour, corn flour and corn for DON recovery. The average recovery was in the range of 77.1% to 107.0%, and the relative standard deviation (RSD) ranged from 4.2% to 11.9%. In addition, the kit has the advantages of high specificity, good stability, a long effective life and negligible sample matrix interference.
Finally, wheat samples from farms in the six provinces of Henan, Anhui, Hebei, Shandong, Jiangsu and Gansu in China were analyzed by the kit. A total of 30 samples were randomly checked (five samples in each province), and the results were in good agreement with the standardized HPLC method. These tests showed that the dcELISA kit had good performance and met relevant technical requirements, and it had the characteristics of accuracy, reliability, convenience and high-throughput screening for DON detection. Therefore, the developed kit is suitable for rapid screening of DON in marketed products.

Characterization of three commercial ELISA kits for detection of BOHV-1 gE specific antibodies in serum and milk samples and applicability of bulk milk for determination of herd status.

Vaccination of animals with gE-deleted vaccine strains (gE- marker vaccines) and differential detection of vaccinated vs infected animals with antibody ELISA targeting the gE or the gB proteins have been proved to be useful tools in programs for control and eradication of the bovine herpesvirus 1 (BoHV-1) responsible for infectious bovine rhinotracheitis (IBR), a major pathogen of cattle.
Multicenter Evaluation of the C6 Lyme ELISA Kit for the Diagnosis of Lyme Disease.
The diagnostic sensitivity (DSe) and specificity (DSp) of three commercial gE ELISA kits from IDEXX, IDVet and CIV-HIPRA were compared for serum and milk matrices. Limiting the analysis to 198 individual with concordant ELISA results in serum (91 naïve, 37 vaccinated and 70 infected) the DSe of gE kits was estimated to 0,97 for IDEXX, 0,93 for CIV-HIPRA and 0,53 for IDVet using milk samples and the DSp to 0,95 for IDEXX, 1,00 for IDVet and CIV-HIPRA.
The applicability of gE ELISA for individual or bulk milk testing as an additional tool in control programs dedicated to the certification and control of vaccinated herds was evaluated. Two of the three evaluated gE ELISA kits presented substantial to good agreement individual milk and serum samples. The bulk-tank milk also proved to be suitable for the detection of BoHV-1 in vaccinated herds provided that gE prevalence is superior to 10% as false negative results are often observed at lower gE herd prevalence. This limitation could be reduced to 8% of prevalence when a prior concentration step was applied to bulk milk samples.

Does serum anti-Müllerian hormone levels always discriminate presence of the ovaries in adult bitches? Comparison of two ELISA kits.

 

Anti-Müllerian hormone (AMH) is produced in the ovary, and thus, it is an excellent marker of follicle pool in females. Current interest is the clinical use of this parameter as a biomarker to assess presence or absence of an intact ovary and to diagnose ovarian remnant syndrome (ORS) following incomplete ovariohysterectomy (OHE) in bitches.
The aim of this study was to evaluate serum AMH concentrations in bitches (n = 34) before and after OHE using two different commercial ELISA kits, one of which is based on detecting human AMH and the other is based on detecting human AMH and the other specified for canine AMH. Furthermore, serum AMH levels were also measured in six ORS cases to compare the diagnostic utility of the two different ELISA kits.
Serum AMH concentrations measured using the human and canine kit prior to and after OHE were 0.32 ± 0.24, 0.006 ± 0.22 ng/ml (p < .001) and 12.08 ± 22.81, 9.55 ± 15.42 ng/ml (p = .868), respectively. Thus, the canine-based kit was not able to reveal the significant drop in serum AMH levels. In conclusion, the human-based ELISA kits successfully detected the drop in serum AMH concentrations.

Rat Lep(Leptin) ELISA Kit

ER0115 96T
EUR 476.25
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.094 ng/ml

Recombinant Rat Leptin

P0193 100ug
EUR 522.36
Description: Recombinant Rat protein for Leptin

Recombinant Mouse Leptin

P0191 100ug
EUR 522.36
Description: Recombinant Mouse protein for Leptin

Human Leptin R(Leptin receptor) ELISA Kit

EH0217 96T
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml

Rat LEPR(Leptin Receptor) ELISA Kit

ER1122 96T
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 18.75pg/ml

Rat Neural Stem Cell Kits

PC37123 1 X 10(6) *minimum order 6 vials*
EUR 957

Hamster Lep(Leptin) ELISA Kit

EHA0011 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Hamster;Sensitivity: 4.688pg/ml

Monkey LEP(Leptin) ELISA Kit

EMK0046 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Ape;Sensitivity: 18.75pg/ml

Porcine LEP(Leptin) ELISA Kit

EP0103 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Pig;Sensitivity: 3.75pg/ml

Bovine Lep(Leptin) ELISA Kit

EB0093 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Cattle;Sensitivity: 0.188 ng/ml

Chicken LEP(Leptin) ELISA Kit

ECH0079 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Chicken;Sensitivity: 46.875pg/ml

Human LEP(Leptin) ELISA Kit

EH0216 96T
EUR 476.25
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml

Rabbit LEP(Leptin) ELISA Kit

ERB0072 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rabbit;Sensitivity: 0.094 ng/ml

Sheep LEP(Leptin) ELISA Kit

ESH0050 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Sheep;Sensitivity: 18.75pg/ml

anti- leptin antibody

FNab04754 100µg
EUR 585
Description: Antibody raised against leptin

Recombinant Human leptin

P0021 100ug
EUR 522.36
Description: Recombinant Human protein for leptin

Recombinant Bovine Leptin

P0187 100ug
EUR 522.36
Description: Recombinant Bovine protein for Leptin

Recombinant Chicken Leptin

P0188 100ug
EUR 522.36
Description: Recombinant Chicken protein for Leptin

Recombinant Dog Leptin

P0189 100ug
EUR 522.36
Description: Recombinant Dog protein for Leptin

Recombinant Human leptin

P0190 100ug
EUR 522.36
Description: Recombinant Human protein for leptin

Recombinant Pig Leptin

P0192 100ug
EUR 522.36
Description: Recombinant Pig protein for Leptin

T&A Cloning Kits

FYC001-20P 1 vial Ask for price

Necrosis vs Apoptosis Kits

KF17371 50-100 Tests
EUR 435

Necrosis vs Apoptosis Kits

KF17372 100-200 Tests
EUR 768

Pipette Stand Starter Kits

P7700-S1 1 PC
EUR 507.83

Pipette Stand Starter Kits

P7700-S2 1 PC
EUR 526.1

Monkey LEPR(Leptin Receptor) ELISA Kit

EMK0047 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Ape;Sensitivity: 0.094 ng/ml

Rat Leptin ELISA Kit

ERL0045 96Tests
EUR 521

Rat Leptin ELISA Kit

ELA-E0084r 96 Tests
EUR 886

Leptin (Rat) ELISA Kit

E4655-100
EUR 729

Rat Leptin ELISA kit

E02L0038-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Leptin ELISA kit

E02L0038-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Leptin ELISA kit

E02L0038-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Leptin ELISA kit

55R-1953 96 tests
EUR 617
Description: ELISA Kit for detection of Leptin in the research laboratory

Leptin, Rat

HY-P7233 50ug
EUR 153

Leptin-Rat

PR27100 200 ug
EUR 191

Mouse Leptin ELISA Kit

EML0045 96Tests
EUR 521

Leptin ELISA Kit| Mouse

EF012889 96 Tests
EUR 689

Leptin (Mouse) ELISA Kit

E4654-100
EUR 729

Mouse Leptin ELISA kit

E03L0038-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Mouse Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin ELISA kit

E03L0038-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Mouse Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin ELISA kit

E03L0038-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin ELISA Kit

E-90LPT 1 x 96 well plate
EUR 441

Mouse Leptin ELISA Kit

55R-1636 1 kit
EUR 550
Description: ELISA kit for detection of Leptin in the research laboratory

Mouse Leptin ELISA kit

55R-2027 96 tests
EUR 617
Description: ELISA Kit for detection of Leptin in the research laboratory

Leptin-Mouse

PR27099 200 ug
EUR 191

Leptin R ELISA Kit| Mouse Leptin Receptor ELISA Kit

EF012890 96 Tests
EUR 689

MitoPT™ TMRE & TMRM Kits

KF17358 500 Test
EUR 479

MitoPT™ TMRE & TMRM Kits

KF17359 500 Test
EUR 479

HISTAR DETECTION SYSTEM Kits (OKSA11259)

OKSA11259 50 Tests
EUR 565
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

HISTAR DETECTION SYSTEM Kits (OKSA11260)

OKSA11260 150 Tests
EUR 1335
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

HISTAR DETECTION SYSTEM Kits (OKSA11261)

OKSA11261 500 Tests
EUR 2019
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol)

RAT-5 1
EUR 1138

Cattle and Goat Hydatid Antibody Rapid Test Kit

abx092072-40tests 40 tests
EUR 356

Rat Leptin(LEP)ELISA ki

GA-E0569RT-48T 48T
EUR 317

Rat Leptin(LEP)ELISA ki

GA-E0569RT-96T 96T
EUR 496

Rat Lep/ Leptin ELISA Kit

E0559Ra 1 Kit
EUR 546

Rat Leptin (LEP) ELISA Kit

abx255813-96tests 96 tests
EUR 668

Rat Leptin (LEP) ELISA Kit

20-abx155779
  • EUR 5374.00
  • EUR 2868.00
  • EUR 676.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Leptin (LEP) ELISA Kit

DLR-LEP-Ra-48T 48T
EUR 508
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leptin (LEP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Leptin (LEP) ELISA Kit

DLR-LEP-Ra-96T 96T
EUR 661
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leptin (LEP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Leptin (LEP) ELISA Kit

RDR-LEP-Ra-48Tests 48 Tests
EUR 534

Rat Leptin (LEP) ELISA Kit

RDR-LEP-Ra-96Tests 96 Tests
EUR 742

Rat Leptin (LEP) ELISA Kit

RD-LEP-Ra-48Tests 48 Tests
EUR 511

Rat Leptin (LEP) ELISA Kit

RD-LEP-Ra-96Tests 96 Tests
EUR 709

Rat Leptin ELISA Kit (LEP)

RK03790 96 Tests
EUR 521

ELISA kit for Rat Leptin

EK1232 96 tests
EUR 469
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Leptin in samples from serum, plasma, tissue homogenates and other biological fluids.

Rat Leptin, LEP ELISA kit

CSB-E07433r-24T 1 plate of 24 wells
EUR 165
Description: Quantitativesandwich ELISA kit for measuring Rat Leptin, LEP in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat Leptin, LEP ELISA kit

1-CSB-E07433r
  • EUR 500.00
  • EUR 3402.00
  • EUR 1820.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat Leptin, LEP in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Leptin (LEP) ELISA Kit

SEA084Ra-10x96wellstestplate 10x96-wells test plate
EUR 3213.06
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Rat Leptin (LEP) ELISA Kit

SEA084Ra-1x48wellstestplate 1x48-wells test plate
EUR 352.25
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Rat Leptin (LEP) ELISA Kit

SEA084Ra-1x96wellstestplate 1x96-wells test plate
EUR 460.36
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Rat Leptin (LEP) ELISA Kit

SEA084Ra-5x96wellstestplate 5x96-wells test plate
EUR 1771.62
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Rat Leptin (LEP) ELISA Kit

4-SEA084Ra
  • EUR 3264.00
  • EUR 1722.00
  • EUR 461.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Leptin (LEP) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Rat Leptin(LEP)ELISA ki

QY-E11083 96T
EUR 361

Rat Leptin,LEP ELISA kit

CN-01503R1 96T
EUR 442

Rat Leptin,LEP ELISA kit

CN-01503R2 48T
EUR 293

Rat Leptin Antibody ELISA kit

E02L0039-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Leptin Antibody ELISA kit

E02L0039-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Leptin Antibody ELISA kit

E02L0039-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Leptin receptor ELISA kit

E02L0273-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Leptin receptor ELISA kit

E02L0273-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Leptin receptor ELISA kit

E02L0273-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin (LEP) ELISA Kit

20-abx154317
  • EUR 5374.00
  • EUR 2868.00
  • EUR 676.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Leptin (LEP) ELISA Kit

abx570297-96tests 96 tests
EUR 731

Mouse Leptin, Lep ELISA Kit

DEIA130 10 plates
EUR 2495
Description: Mouse Leptin ELISA development kit contains the key components required for the quantitative measurement of natural and/or recombinant Leptin in a sandwich ELISA format within the range of 20-1,250 pg/mL. Using the ELISA protocol described below, this kit provides sufficient reagents to assay Leptin in approximately 1,000 ELISA plate wells.

Mouse Leptin(LEP)ELISA Kit        

GA-E0665MS-48T 48T
EUR 336

Mouse Leptin(LEP)ELISA Kit        

GA-E0665MS-96T 96T
EUR 534

Mouse Lep/ Leptin ELISA Kit

E0859Mo 1 Kit
EUR 546

Mouse Leptin (LEP) ELISA Kit

abx050143-96tests 96 tests
EUR 668

Mouse Leptin (LEP) ELISA Kit

abx051335-96tests 96 tests
EUR 754

Mouse Leptin (LEP) ELISA Kit

abx254349-96tests 96 tests
EUR 731
Reliable results can only be achieved from well-designed ELISA kits, and AMH levels might be a useful diagnostic tool for the evaluation of presence or absence of ovaries as well as for the detection of ORS cases in bitches.