Lyme disease (LD), caused by infection with Borrelia burgdorferi, is the most common tick-borne infection in many regions of Eurasia. Antibody detection is the most frequently used laboratory test, favoring a two-step serodiagnostic algorithm; immunoenzymatic detection of antibodies to C6 has been shown to perform similarly to a standard two-step workflow.
The aim of this study was the performance evaluation of the C6 Lyme ELISA kit compared to a standard two-step algorithm in three laboratories located in the northeastern region of Italy which cater to areas with different LD epidemiology. A total of 804 samples were tested, of which 695 gave concordant results between C6 testing and routine workflow (564 negative, 131 positive).
Wherever available, clinical presentation and additional laboratory tests were analyzed to solve discrepancies. The C6 based method showed a good concordance with the standard two-step algorithm (Cohen’s κ = 0.619), however, the distribution of discrepancies seems to point towards a slightly lower specificity of C6 testing, which is supported by literature and could impact on patient management. The C6 ELISA, therefore, is not an ideal stand-alone test; however, if integrated into a two-step algorithm, it might play a part in achieving a sensitive, specific laboratory diagnosis of LD.
Development of a Direct Competitive ELISA Kit for Detecting Deoxynivalenol Contamination in Wheat.
This study was conducted to develop a self-assembled direct competitive enzyme-linked immunosorbent assay (dcELISA) kit for the detection of deoxynivalenol (DON) in food and feed grains. Based on the preparation of anti-DON monoclonal antibodies, we established a standard curve with dcELISA and optimized the detection conditions. The performance of the kit was evaluated by comparison with high-performance liquid chromatography (HPLC).
The minimum detection limit of DON with the kit was 0.62 ng/mL, the linear range was from 1.0 to 113.24 ng/mL and the half-maximal inhibition concentration (IC50) was 6.61 ng/mL in the working buffer; there was a limit of detection (LOD) of 62 ng/g, and the detection range was from 100 to 11324 ng/g in authentic agricultural samples. We examined four samples of wheat bran, wheat flour, corn flour and corn for DON recovery. The average recovery was in the range of 77.1% to 107.0%, and the relative standard deviation (RSD) ranged from 4.2% to 11.9%. In addition, the kit has the advantages of high specificity, good stability, a long effective life and negligible sample matrix interference.
Finally, wheat samples from farms in the six provinces of Henan, Anhui, Hebei, Shandong, Jiangsu and Gansu in China were analyzed by the kit. A total of 30 samples were randomly checked (five samples in each province), and the results were in good agreement with the standardized HPLC method. These tests showed that the dcELISA kit had good performance and met relevant technical requirements, and it had the characteristics of accuracy, reliability, convenience and high-throughput screening for DON detection. Therefore, the developed kit is suitable for rapid screening of DON in marketed products.
Characterization of three commercial ELISA kits for detection of BOHV-1 gE specific antibodies in serum and milk samples and applicability of bulk milk for determination of herd status.
Vaccination of animals with gE-deleted vaccine strains (gE- marker vaccines) and differential detection of vaccinated vs infected animals with antibody ELISA targeting the gE or the gB proteins have been proved to be useful tools in programs for control and eradication of the bovine herpesvirus 1 (BoHV-1) responsible for infectious bovine rhinotracheitis (IBR), a major pathogen of cattle.
The diagnostic sensitivity (DSe) and specificity (DSp) of three commercial gE ELISA kits from IDEXX, IDVet and CIV-HIPRA were compared for serum and milk matrices. Limiting the analysis to 198 individual with concordant ELISA results in serum (91 naïve, 37 vaccinated and 70 infected) the DSe of gE kits was estimated to 0,97 for IDEXX, 0,93 for CIV-HIPRA and 0,53 for IDVet using milk samples and the DSp to 0,95 for IDEXX, 1,00 for IDVet and CIV-HIPRA.
The applicability of gE ELISA for individual or bulk milk testing as an additional tool in control programs dedicated to the certification and control of vaccinated herds was evaluated. Two of the three evaluated gE ELISA kits presented substantial to good agreement individual milk and serum samples. The bulk-tank milk also proved to be suitable for the detection of BoHV-1 in vaccinated herds provided that gE prevalence is superior to 10% as false negative results are often observed at lower gE herd prevalence. This limitation could be reduced to 8% of prevalence when a prior concentration step was applied to bulk milk samples.
Does serum anti-Müllerian hormone levels always discriminate presence of the ovaries in adult bitches? Comparison of two ELISA kits.
Anti-Müllerian hormone (AMH) is produced in the ovary, and thus, it is an excellent marker of follicle pool in females. Current interest is the clinical use of this parameter as a biomarker to assess presence or absence of an intact ovary and to diagnose ovarian remnant syndrome (ORS) following incomplete ovariohysterectomy (OHE) in bitches.
The aim of this study was to evaluate serum AMH concentrations in bitches (n = 34) before and after OHE using two different commercial ELISA kits, one of which is based on detecting human AMH and the other is based on detecting human AMH and the other specified for canine AMH. Furthermore, serum AMH levels were also measured in six ORS cases to compare the diagnostic utility of the two different ELISA kits.
Serum AMH concentrations measured using the human and canine kit prior to and after OHE were 0.32 ± 0.24, 0.006 ± 0.22 ng/ml (p < .001) and 12.08 ± 22.81, 9.55 ± 15.42 ng/ml (p = .868), respectively. Thus, the canine-based kit was not able to reveal the significant drop in serum AMH levels. In conclusion, the human-based ELISA kits successfully detected the drop in serum AMH concentrations.
Rat Lep(Leptin) ELISA Kit |
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| ER0115 | 96T | EUR 476.25 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.094 ng/ml |
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Recombinant Rat Leptin |
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| P0193 | 100ug | EUR 522.36 |
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Description: Recombinant Rat protein for Leptin |
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Recombinant Mouse Leptin |
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| P0191 | 100ug | EUR 522.36 |
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Description: Recombinant Mouse protein for Leptin |
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Human Leptin R(Leptin receptor) ELISA Kit |
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| EH0217 | 96T | EUR 524.1 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml |
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Rat LEPR(Leptin Receptor) ELISA Kit |
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| ER1122 | 96T | EUR 524.1 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 18.75pg/ml |
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Rat Neural Stem Cell Kits |
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| PC37123 | 1 X 10(6) *minimum order 6 vials* | EUR 957 |
Hamster Lep(Leptin) ELISA Kit |
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| EHA0011 | 96T | EUR 567.6 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Hamster;Sensitivity: 4.688pg/ml |
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Monkey LEP(Leptin) ELISA Kit |
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| EMK0046 | 96T | EUR 567.6 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Ape;Sensitivity: 18.75pg/ml |
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Porcine LEP(Leptin) ELISA Kit |
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| EP0103 | 96T | EUR 567.6 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Pig;Sensitivity: 3.75pg/ml |
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Bovine Lep(Leptin) ELISA Kit |
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| EB0093 | 96T | EUR 567.6 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Cattle;Sensitivity: 0.188 ng/ml |
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Chicken LEP(Leptin) ELISA Kit |
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| ECH0079 | 96T | EUR 567.6 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Chicken;Sensitivity: 46.875pg/ml |
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Human LEP(Leptin) ELISA Kit |
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| EH0216 | 96T | EUR 476.25 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml |
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Rabbit LEP(Leptin) ELISA Kit |
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| ERB0072 | 96T | EUR 567.6 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rabbit;Sensitivity: 0.094 ng/ml |
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Sheep LEP(Leptin) ELISA Kit |
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| ESH0050 | 96T | EUR 567.6 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Sheep;Sensitivity: 18.75pg/ml |
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anti- leptin antibody |
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| FNab04754 | 100µg | EUR 585 |
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Description: Antibody raised against leptin |
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Recombinant Human leptin |
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| P0021 | 100ug | EUR 522.36 |
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Description: Recombinant Human protein for leptin |
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Recombinant Bovine Leptin |
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| P0187 | 100ug | EUR 522.36 |
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Description: Recombinant Bovine protein for Leptin |
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Recombinant Chicken Leptin |
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| P0188 | 100ug | EUR 522.36 |
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Description: Recombinant Chicken protein for Leptin |
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Recombinant Dog Leptin |
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| P0189 | 100ug | EUR 522.36 |
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Description: Recombinant Dog protein for Leptin |
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Recombinant Human leptin |
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| P0190 | 100ug | EUR 522.36 |
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Description: Recombinant Human protein for leptin |
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Recombinant Pig Leptin |
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| P0192 | 100ug | EUR 522.36 |
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Description: Recombinant Pig protein for Leptin |
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T&A Cloning Kits |
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| FYC001-20P | 1 vial | Ask for price |
Necrosis vs Apoptosis Kits |
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| KF17371 | 50-100 Tests | EUR 435 |
Necrosis vs Apoptosis Kits |
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| KF17372 | 100-200 Tests | EUR 768 |
Pipette Stand Starter Kits |
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| P7700-S1 | 1 PC | EUR 507.83 |
Pipette Stand Starter Kits |
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| P7700-S2 | 1 PC | EUR 526.1 |
Monkey LEPR(Leptin Receptor) ELISA Kit |
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| EMK0047 | 96T | EUR 567.6 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Ape;Sensitivity: 0.094 ng/ml |
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Rat Leptin ELISA Kit |
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| ERL0045 | 96Tests | EUR 521 |
Rat Leptin ELISA Kit |
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| ELA-E0084r | 96 Tests | EUR 886 |
Leptin (Rat) ELISA Kit |
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| E4655-100 | EUR 729 | |
Rat Leptin ELISA kit |
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| E02L0038-192T | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Rat Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Leptin ELISA kit |
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| E02L0038-48 | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Rat Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Leptin ELISA kit |
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| E02L0038-96 | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Rat Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Leptin ELISA kit |
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| 55R-1953 | 96 tests | EUR 617 |
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Description: ELISA Kit for detection of Leptin in the research laboratory |
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Leptin, Rat |
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| HY-P7233 | 50ug | EUR 153 |
Leptin-Rat |
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| PR27100 | 200 ug | EUR 191 |
Mouse Leptin ELISA Kit |
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| EML0045 | 96Tests | EUR 521 |
Leptin ELISA Kit| Mouse |
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| EF012889 | 96 Tests | EUR 689 |
Leptin (Mouse) ELISA Kit |
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| E4654-100 | EUR 729 | |
Mouse Leptin ELISA kit |
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| E03L0038-192T | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Mouse Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Leptin ELISA kit |
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| E03L0038-48 | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Mouse Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Leptin ELISA kit |
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| E03L0038-96 | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Mouse Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Leptin ELISA Kit |
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| E-90LPT | 1 x 96 well plate | EUR 441 |
Mouse Leptin ELISA Kit |
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| 55R-1636 | 1 kit | EUR 550 |
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Description: ELISA kit for detection of Leptin in the research laboratory |
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Mouse Leptin ELISA kit |
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| 55R-2027 | 96 tests | EUR 617 |
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Description: ELISA Kit for detection of Leptin in the research laboratory |
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Leptin-Mouse |
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| PR27099 | 200 ug | EUR 191 |
Leptin R ELISA Kit| Mouse Leptin Receptor ELISA Kit |
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| EF012890 | 96 Tests | EUR 689 |
MitoPT™ TMRE & TMRM Kits |
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| KF17358 | 500 Test | EUR 479 |
MitoPT™ TMRE & TMRM Kits |
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| KF17359 | 500 Test | EUR 479 |
HISTAR DETECTION SYSTEM Kits (OKSA11259) |
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| OKSA11259 | 50 Tests | EUR 565 |
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Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity: |
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HISTAR DETECTION SYSTEM Kits (OKSA11260) |
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| OKSA11260 | 150 Tests | EUR 1335 |
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Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity: |
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HISTAR DETECTION SYSTEM Kits (OKSA11261) |
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| OKSA11261 | 500 Tests | EUR 2019 |
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Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity: |
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Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol) |
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| RAT-5 | 1 | EUR 1138 |
Cattle and Goat Hydatid Antibody Rapid Test Kit |
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| abx092072-40tests | 40 tests | EUR 356 |
Rat Leptin(LEP)ELISA ki |
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| GA-E0569RT-48T | 48T | EUR 317 |
Rat Leptin(LEP)ELISA ki |
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| GA-E0569RT-96T | 96T | EUR 496 |
Rat Lep/ Leptin ELISA Kit |
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| E0559Ra | 1 Kit | EUR 546 |
Rat Leptin (LEP) ELISA Kit |
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| abx255813-96tests | 96 tests | EUR 668 |
Rat Leptin (LEP) ELISA Kit |
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| 20-abx155779 |
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Rat Leptin (LEP) ELISA Kit |
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| DLR-LEP-Ra-48T | 48T | EUR 508 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat Leptin (LEP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Rat Leptin (LEP) ELISA Kit |
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| DLR-LEP-Ra-96T | 96T | EUR 661 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat Leptin (LEP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Rat Leptin (LEP) ELISA Kit |
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| RDR-LEP-Ra-48Tests | 48 Tests | EUR 534 |
Rat Leptin (LEP) ELISA Kit |
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| RDR-LEP-Ra-96Tests | 96 Tests | EUR 742 |
Rat Leptin (LEP) ELISA Kit |
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| RD-LEP-Ra-48Tests | 48 Tests | EUR 511 |
Rat Leptin (LEP) ELISA Kit |
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| RD-LEP-Ra-96Tests | 96 Tests | EUR 709 |
Rat Leptin ELISA Kit (LEP) |
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| RK03790 | 96 Tests | EUR 521 |
ELISA kit for Rat Leptin |
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| EK1232 | 96 tests | EUR 469 |
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Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Leptin in samples from serum, plasma, tissue homogenates and other biological fluids. |
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Rat Leptin, LEP ELISA kit |
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| CSB-E07433r-24T | 1 plate of 24 wells | EUR 165 |
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Description: Quantitativesandwich ELISA kit for measuring Rat Leptin, LEP in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
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Rat Leptin, LEP ELISA kit |
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| 1-CSB-E07433r |
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Description: Quantitativesandwich ELISA kit for measuring Rat Leptin, LEP in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
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Rat Leptin (LEP) ELISA Kit |
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| SEA084Ra-10x96wellstestplate | 10x96-wells test plate | EUR 3213.06 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
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Rat Leptin (LEP) ELISA Kit |
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| SEA084Ra-1x48wellstestplate | 1x48-wells test plate | EUR 352.25 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
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Rat Leptin (LEP) ELISA Kit |
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| SEA084Ra-1x96wellstestplate | 1x96-wells test plate | EUR 460.36 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
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Rat Leptin (LEP) ELISA Kit |
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| SEA084Ra-5x96wellstestplate | 5x96-wells test plate | EUR 1771.62 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
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Rat Leptin (LEP) ELISA Kit |
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| 4-SEA084Ra |
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Leptin (LEP) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. |
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Rat Leptin(LEP)ELISA ki |
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| QY-E11083 | 96T | EUR 361 |
Rat Leptin,LEP ELISA kit |
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| CN-01503R1 | 96T | EUR 442 |
Rat Leptin,LEP ELISA kit |
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| CN-01503R2 | 48T | EUR 293 |
Rat Leptin Antibody ELISA kit |
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| E02L0039-192T | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Rat Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Leptin Antibody ELISA kit |
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| E02L0039-48 | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Rat Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Leptin Antibody ELISA kit |
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| E02L0039-96 | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Rat Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Leptin receptor ELISA kit |
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| E02L0273-192T | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Rat Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Leptin receptor ELISA kit |
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| E02L0273-48 | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Rat Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Leptin receptor ELISA kit |
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| E02L0273-96 | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Rat Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Leptin (LEP) ELISA Kit |
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| 20-abx154317 |
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Mouse Leptin (LEP) ELISA Kit |
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| abx570297-96tests | 96 tests | EUR 731 |
Mouse Leptin, Lep ELISA Kit |
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| DEIA130 | 10 plates | EUR 2495 |
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Description: Mouse Leptin ELISA development kit contains the key components required for the quantitative measurement of natural and/or recombinant Leptin in a sandwich ELISA format within the range of 20-1,250 pg/mL. Using the ELISA protocol described below, this kit provides sufficient reagents to assay Leptin in approximately 1,000 ELISA plate wells. |
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Mouse Leptin(LEP)ELISA Kit |
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| GA-E0665MS-48T | 48T | EUR 336 |
Mouse Leptin(LEP)ELISA Kit |
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| GA-E0665MS-96T | 96T | EUR 534 |
Mouse Lep/ Leptin ELISA Kit |
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| E0859Mo | 1 Kit | EUR 546 |
Mouse Leptin (LEP) ELISA Kit |
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| abx050143-96tests | 96 tests | EUR 668 |
Mouse Leptin (LEP) ELISA Kit |
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| abx051335-96tests | 96 tests | EUR 754 |
Mouse Leptin (LEP) ELISA Kit |
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| abx254349-96tests | 96 tests | EUR 731 |
Reliable results can only be achieved from well-designed ELISA kits, and AMH levels might be a useful diagnostic tool for the evaluation of presence or absence of ovaries as well as for the detection of ORS cases in bitches.